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Tetrodotoxin (TTX) is a powerful neurotoxin, which is tolerance to heat, salt and cooking .Minimum lethal dose for human is about 0.5mg/60 kg of body weight, The toxicity is 1000 times great than the sodium cyanide. Every year many people are ill due to improper eating or eating puffer fish.Therefore, it is significance to accurate detection of tetrodotoxin in puffer fish in order to prevention and control of tetrodotoxin poisoning . The detection method is sensitive, fast, simple and specific, only need a small amount of fish sampling .

Beta-lactam Antibiotics Penicillins Cefalosporins Antibiotic (MRL) Detection limit (¦Ìg/L) Antibiotic (MRL) Detection limit (¦Ìg/L) Penicillin G (4) 3-4 ppb Ceftiofur (100) 75-100 ppb Ampicillin (4) 4-5 ppb Cephapirin (60) 6-15 ppb Amoxicillin (4) 4-5 ppb Cefazolin (50) 35-50 ppb Cloxacillin (30) 4-8 ppb Cefoperazone(50) 5-20 ppb Oxacillin (30) 4-8 ppb Cephquinome (20) 10-20 ppb Dicloxacillin (30) 5-8 ppb Cephalonium (10) 4-8 ppb Nafcillin (30) 15-30 ppb Sulfonamide Antibiotics Tetracycline Antibiotics Antibiotic (MRL) Detection limit (¦Ìg/L) Antibiotic (MRL) Detection limit (¦Ìg/L) Sulfmethazine (100) 25-50 ppb Tetracycline (100) 50-100 ppb Sulfamethoxazole (100) 25-50 ppb Chlorotetracycline (100) 15-50 ppb Sulfadiazine (100) 50-75 ppb Doxycycline (100) 5-20 ppb Sulfathiazole (100) 75-100 ppb Oxytetracycline (100) 50-70 ppb Sulfadimethoxine (100) 50-75 ppb Sulfaquinoxaline (100) 75-100 ppb Sulfapyridine (100) 2-4 ppb Sulfamethoxypyridazine (100) 3-4 ppb Sulfamethizole (100) 40-50 ppb Sulfamerazine (100) 25-50 ppb Sulfachloropyridazine (100) 25-50 ppb

Sample Type Detection Limit (ng/g or ppb) Mussels 3 Water 1

Beta-lactam Antibiotics Tetracycline Antibiotics Penicillins Cefalosporins Antibiotic (MRL) Detectionli mit( ppb) Antibiotic (MRL) Detection limit (ppb) Antibiotic (MRL) Detection limit ppb) Penicillin (4) 3-4 Ceftiofur (100) 75-100 Tetracycline (100) 50-75 Ampicillin (4) 4-5 Cephapirin (60) 6-15 Chlorotetracycline (100) 20-50 Amoxicillin (4) 4-5 Cefazolin (50) 35-50 Doxycycline (100) 10-35 Cloxacillin (30) 4-8 Cefoperazone (50) 5-20 Oxytetracycline (100) 30-55 Oxacillin (30) 4-8 Cephquinome (20) 10-20 Dicloxacillin (30) 5-8 Cephalonium (10) 3-10 Nafcillin (30) 15-30

Sample Type Detection Limit (ng/g or ppb) Feed 0..1 Fish/Shrimp/Meat 0.05 Egg/Egg Powder 0.05 Honey 0.05 Milk 0.05 Serum 0.05 Urine 0.05